It has been 30 years since the invention of PCR (Polymerase Chain Reaction). During the 30 years, PCR technology has been improved by numerous scholars continuously and now it has become the most widely, frequently used and important research tool. Based on the wide application range of traditional PCR technology, Touch Down PCR, RT-PCR, Real-Time PCR, Multi PCR and the latest emerging technology---Digital PCR has greatly enriched technical methods for scientific researchers, and accelerated the development of modern life sciences, especially in terms of molecular biology.
Conventional PCR technique, as well as new technologies and applications derived from it, requires high-purity nucleic acid templates to be obtained in advance, which indicates that all samples need to be processed through a series of complex and cumbersome steps to meet the requirements. Steps to separate and extract DNA or RNA have been always repeated by researchers, who have enough technical proficiency in dealing with different experimental samples. But there is one thing to pay attention that some strong toxic chemicals are used in conventional separation and extraction techniques, which would cause irreversible damage to operators after long-term exposure and even direct damage in an experiment. It has been proven that some chemical agents, especially phenol chloroform and liquid nitrogen, are carcinogen in laboratory. The latter one has been frequently used in the nucleic acids isolation and extraction from plant tissues and it is a great threat for operators’ safety due to its ultra-low temperature.
What’s more, it is an enormous task for researchers to separate and extract nucleic acids when studying a large number of samples. Nowadays nucleic acids isolation and extraction kits of different brands with mature protocols and similar functions, have been everywhere in the marketplace. Either silicone membrane column centrifugal kit or magnetic bead kit is time-consuming and costly. Besides, there are some special requirements for laboratory equipments, just like the automated workstation required in magnetic bead way is typically a set of high-value equipment, which is a huge expense for a laboratory team.
In a word, in preparation for PCR related experiments, samples pretreatment are unavoidable and difficult tasks. A majority of researchers and clinical laboratory staff have been thinking how to solve this problem and if the PCR experiments can be run without isolation and extraction of nucleic acids.
Creative Biogene has developed Direct PCR Kits for various samples through several years of painstaking research. In spite of numerous limitations, our professional researchers have succeeded in making a great breakthrough with strong resistance and adaptability. These new developments have greatly reduced labor intensity, promoted experimental progress, and saved the research and test expenditure.
Followings are the understanding and awareness about Direct PCR from Creative Biogene
- Direct PCR technique is designedfor all types of biological tissue samples. With this technique, researchers can run PCR reactions by directly taking samples as templates and adding specific primers to obtain target genes, without nucleic acids isolation and extraction previously.
- Direct PCR technology is not just atraditional technique for DNA amplification, but also for reverse transcription PCR when RNA templates exist.
- Direct PCR technology is notonly used for conventional PCR analysis qualitatively, but also for Real-Time qPCR analysis quantitatively, which requires the reaction system with strong anti-interference ability to background fluorescence and antagonistic ability to endogenous fluorescence quencher.
- Fordifferent tissue samples, the only prerequisite for Direct PCR technique is the release of nucleic acid templates. There is no need to remove proteins, polysaccharides and ions salt, which might interfere in PCR reactions. It means that nucleic acid polymerase and PCR Mix in various reaction systems have excellent resistance and adaptability, to ensure accurate replication and enzyme activity under complex conditions.
- Tissue samplesprocessed by Direct PCR technique are without any nucleic acids-enriching treatment. In other words, it has a minimal amount of templates in an initial reaction system, which requires the functional reagents provided by Direct PCR kit should have high sensitivity and amplification efficiency.
Direct PCR technique and its related kits from Creative Biogene are in full compliance with the requirements above. It can simplify the preparation process before PCR experiments, and make it easy and quick to get results of what we want.
For 30 years, Direct PCR is one of the most important development and innovation for PCR technology. Creative Biogene has been continuing to be a leader and innovator in this field. Our featured products are Blood Direct PCR Kit, Mouse Tail Direct PCR Kit and Cell Direct PCR Kit. The application of Direct PCR has a promising future. Its continuous improvement and universal application will bring great change for research and inspection work. This is definitely a PCR technology revolution.