Cat.No. : CSC-SC014485-1 Host Cell: CHO-K1
| Cat. No. | CSC-SC014485-1 |
| Description | This cell line is engineered to stably express human solute carrier family 2 member 3 (SLC2A3) in CHO-K1 cells. |
| Gene | SLC2A3 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Clear cell renal cell carcinoma (ccRCC) is the most common subtype of RCC. Researchers used the SLC2A3 cell line to explore the regulatory mechanism of LINC01094 in the development of clear cell renal cell carcinoma (ccRCC). The targeting relationship between miR-184, LINC01094, and SLC2A3 was verified through dual-luciferase reporter experiments. RNA immunoprecipitation (RIP) and RNA pull-down experiments further confirmed the interaction between LINC01094 and miR-184. The biological behavior of ccRCC cells was studied through cell counting kit-8 (CCK8), scratch assay, Transwell assay, and flow cytometry. In in vivo experiments, the effect of SLC2A3 on tumor formation ability in nude mice was evaluated. The results showed that LINC01094 and SLC2A3 were highly expressed, while miR-184 was low expressed in ccRCC cells and clinical tissues. miR-184 was verified as a direct target of LINC01094. Silencing LINC01094, upregulating miR-184, or reducing SLC2A3 inhibited the growth, migration, and invasion of ccRCC cells. Silencing LINC01094 reduces the expression of SLC2A3 by upregulating miR-184, thereby inhibiting the development of ccRCC.
Figure 1. The researchers used the SLC2A3-related cell line (SLC2A3 gene silencing cell line, SLC2A3 gene overexpression cell line) and used bioinformatics prediction, dual-luciferase reporter gene, microarray, Western Blot, immunohistochemistry, RT-qPCR, CCK-8, scratch assay, Transwell, flow cytometry, and other methods to analyze the regulatory relationship between miR-184 and SLC2A3 in clear cell renal cell carcinoma. (Xu H, et al., 2020)
A: Human SLC2A3 Stable Cell Line-CHOK1 has significant advantages in glucose metabolism research because SLC2A3 (also known as GLUT3) is a high-affinity glucose transporter that is mainly responsible for the transport of glucose across the membrane. This makes this cell line particularly suitable for studying glucose uptake and metabolic pathways in cells. In addition, the CHOK1 cell line itself has been widely used in metabolic research and is highly operable and reproducible.
A: Several methods can be used to ensure functional expression of SLC2A3. First, qPCR and Western Blotting were used to confirm the mRNA and protein levels of SLC2A3. Second, the functional activity of SLC2A3 is assessed by glucose uptake experiments (e.g., using radioactively labeled glucose or fluorescently labeled glucose analogs). In addition, cell growth and survival experiments can be performed to detect the physiological response of cells under different glucose concentrations to further verify the functionality of SLC2A3.
A: The culture conditions of Human SLC2A3 Stable Cell Line-CHOK1 are similar to general CHOK1 cells, but special attention needs to be paid to glucose concentration and culture medium composition to ensure optimal expression and function. It is recommended to use high glucose DMEM medium and appropriately supplement serum and necessary antibiotics to maintain cell growth and stability. In addition, the expression level and function of SLC2A3 need to be regularly tested during the screening and culture process to ensure its stability and activity.
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The cells provided by this supplier on the platform are really good. I observed consistent growth of cells and stable gene expression in culture. Very suitable for my research. The scalability and stability of CHO-K1 cells save me a lot of worry in my experiments. Cell metabolism is also ideal.
Using cells from this company, we found that they maintained a good growth state during the culture process, and their gene expression was also very stable. Especially CHO-K1 cells are easy to operate and suitable for large-scale production, which is very suitable for my project needs. The cells behaved stably in all aspects.
The supplier's CHO-K1 cell line is very reliable. The cells grow consistently during the culture period and the gene expression is also very stable. It is especially suitable for my protein synthesis experiments. Cell metabolism is stable and provides sufficient glucose, which is especially suitable for long-term research work.
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