sgRNA Library
Creative Biogene is one of the leading biotechnology companies in providing sgRNA library service for customers. Our sophisticated equipments, advanced technologies and highly experienced staffs are available to assist you in designing sgRNA constructs to target particular genes, synthesis oligos on arrays and clone the synthesized oligos into lentiviral vectors. With the help of highly experienced staffs, Creative Biogene can promise the fastest turnaround time than any other suppliers in the industry.
A single guide RNA (sgRNA, also known as guide RNA or gRNA) is a RNA sequence that can guide the Cas9 to the desired location of the cell’s genome.[1, 2, 3]With the continuous development of RNA technology, sgRNA library service is available now. Creative Biogene offers sgRNA libraries for high-throughput knockout of specific genes, which is a powerful tool for systematic genetic analysis in mammalian cells, facilitating gene discovery, genome-scale functional interrogation and drug discovery. With years of experience in research of CRISPR/Cas9 system, Creative Biogene can provide you with the best research materials and support in the CRISPR/Cas9 and sgRNA field.
Application:
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High-throughput knockout screening
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Drug target discovery and validation
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Phenotypic screens
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Reporter assays
Feature:
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High-throughput – sgRNA libraries of genome-wide is available
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Strict quality control
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Competitive prices
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Fast turnaround time
Creative Biogene offers custom sgRNA library services for your scientific research as follows:
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Design sgRNA constructs to target the genes.
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Synthesis oligos on arrays.
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Cloning the synthesized oligos into lentiviral vectors.
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Generation of high-titer virus contained the library constructs.
References:
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Ledford H. CRISPR, the disruptor.Nature, 2015, 522(7554):20-4.
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Doench JG, Fusi N, Sullender M, et al. Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016, 34(2):184–191
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HendelA, BakRO, ClarkJT, et al. Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells. Nature biotechnology, 2015.
Frequently Asked Questions of sgRNA library service
* For research use only. Not intended for any clinical use.