Cat.No. : CSC-SC010567-1 Host Cell: HEK293
| Cat. No. | CSC-SC010567-1 |
| Description | This cell line is engineered to stably express human natriuretic peptide receptor 3 (NPR3) in HEK293 cells. |
| Gene | NPR3 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Mice transmit itch via separate neural pathways: one histamine-dependent, the other histamine-independent. These pathways express gastrin-releasing peptide (GRP) and neuromedin B (NMB), respectively, to the spinal cord. Researchers demonstrate that NPR3, encoding the BNP receptor NPRC, plays a crucial role in mediating histaminergic itch transmission. NPR3 expression overlaps with NMBR in the dorsal horn, implicated in histaminergic itch via Gq-coupled signaling. NPRC is required for histamine-induced itch but not non-histaminergic itch. Importantly, BNP enhances scratching behaviors mediated by NMB, not GRP, indicating NPRC-NMBR cross-signaling. These findings unveil a novel mechanism whereby BNP facilitates NMB-encoded itch through NPRC-NMBR interaction, revealing distinct neuropeptide actions in itch modulation.
Figure 1. NPR3's role in the molecular profile of the spinal cord, particularly its co-expression with Nmbr and Vglut2 in laminae I-II, is demonstrated by researchers. (Meng QT, et al., 2021)
The utilization of Creative Biogene's Human NPR3 Stable Cell Line - HEK293 may enhance experimental approaches similar to those described, offering a controlled in vitro system for investigating NPR3-related molecular interactions and signaling pathways.
A: HEK293 cells were likely chosen for their robust expression machinery, allowing for efficient production of membrane-bound proteins like NPR3, essential for studying its role in cardiovascular physiology.
A: Stability was likely confirmed through methods such as immunoblotting, flow cytometry, or functional assays assessing NPR3-mediated cGMP signaling, with continuous selection pressure applied.
A: Characterization may involve analysis of NPR3 localization, ligand-binding kinetics, downstream signaling pathways, and functional implications in blood pressure regulation and cardiovascular homeostasis.
A: Quality control likely included screening for mycoplasma contamination, confirmation of stable transgene integration, and assessment of phenotypic stability and consistency.
A: Comparative analysis with primary cardiomyocytes or animal models helps validate the relevance of NPR3 expression in blood pressure regulation, heart failure, and potential pharmacological targeting for cardiovascular therapies.
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This Human NPR3 Stable Cell Line is a gem! Its stable expression in HEK293 cells has revolutionized my research on natriuretic peptide signaling.
Using this cell line feels like having a secret weapon! The stable NPR3 expression has made studying cardiovascular function pathways a breeze.
Can't believe how much easier my experiments have become with this cell line! Its reliable expression in HEK293 cells has elevated the quality of my research on hypertension.
So impressed with this Human NPR3 Stable Cell Line! Its consistent expression has given me peace of mind and boosted the credibility of my findings.
Huge shoutout to this cell line for simplifying my work! With stable NPR3 expression, I can delve deeper into cardiovascular function without any worries.
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