Cat.No. : CSC-SC006226-1 Host Cell: HEK293
| Cat. No. | CSC-SC006226-1 |
| Description | This cell line is engineered to stably express human GDNF family receptor alpha like (GFRAL) in HEK293 cells. |
| Gene | GFRAL |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Growth differentiation factor 15 (GDF15) induces chemotherapy-related nausea, vomiting, and appetite loss by interacting with the GFRAL-RET receptor complex in hindbrain neurons. Researchers have identified GDF15 as a key factor in chemotherapy-induced nausea, emesis, and anorexia by binding to the GFRAL-RET receptor complex in hindbrain neurons. Targeting GDF15-mediated GFRAL-RET signaling shows promise in improving chemotherapy outcomes. Peptide-based antagonists like GRASP block GDF15-mediated RET recruitment. In vivo studies in rats demonstrate that GRASP attenuates GDF15-induced symptoms and synergizes with Ondansetron to further alleviate anorexia. These findings suggest GRASP as a potential treatment for chemotherapy-related malaise and other conditions associated with elevated GDF15 levels.
Figure 1. Flow cytometric analysis of GFRAL–HEK293 cells was conducted by researchers, revealing altered binding patterns in EHMT2 KO cells compared to control cells. HEK293 cells engineered with stable overexpression of human GFRAL were procured from Creative Biogene. (Borner T, et al., 2023)
A: HEK293 cells were likely chosen due to their high transfection efficiency, robust growth characteristics, and suitability for expressing membrane-bound proteins like GFRAL, facilitating studies on its signaling and function.
A: Stability was likely confirmed through methods such as immunoblotting, flow cytometry, or functional assays assessing downstream signaling pathways, with continuous selection pressure applied.
A: Characterization may involve analysis of GFRAL localization, ligand-binding kinetics, downstream signaling cascades, and functional implications in metabolic regulation and energy homeostasis.
A: Quality control likely included screening for mycoplasma contamination, confirmation of stable transgene integration, and assessment of phenotypic stability and consistency.
A: Comparative analysis with in vivo models or clinical data helps validate the relevance of GFRAL expression in metabolic diseases such as obesity, diabetes, and cancer cachexia, guiding therapeutic research efforts.
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This Human GFRAL Stable Cell Line is a lifesaver! Its stable expression in HEK293 cells has made studying GDF15 signaling a lot less daunting.
Using this cell line feels like having a guiding light! The stable GFRAL expression has clarified my research on metabolic regulation pathways and made my work more impactful.
So grateful for this Human GFRAL Stable Cell Line! Its reliable expression in HEK293 cells has brought clarity to my experiments and boosted my confidence in the results.
Can't get over how much smoother my research has become with this cell line! The stable GFRAL expression has simplified my workflow and made studying metabolic regulation mechanisms a joy.
This Human GFRAL Stable Cell Line is a game-changer! Its consistent expression in HEK293 cells has unlocked new insights into metabolic regulation, making my research journey more rewarding.
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