Cat.No. : AAB0049
Titer: ≥1x10^12 GC/mL / ≥1x10^13 GC/mL Size: 30 ul/100 ul/500 ul/1 ml
Serotype: AAV Serotype 9 Storage: -80 ℃
| Cat. No. | AAB0049 |
| Description | Premade AAV particles in serotype 9 containing Cre-dependent GCaMP6s under the control of a CAG promoter. |
| Serotype | AAV Serotype 9 |
| Tag | GCaMP6s |
| Product Type | Adeno-associated virus particles |
| Biosensor | GCaMP6s-Improved SNR, slower kinetics; Green indicator |
| Titer | Varies lot by lot, typically ≥1x10^12 GC/mL |
| Size | Varies lot by lot, for example, 30 μL, 50 μL, 100 μL etc. |
| Storage | Store at -80℃. Avoid multiple freeze/thaw cycles. |
| Shipping | Frozen on dry ice |
| Creative Biogene ensures high-quality AAV particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between AAV particle lots. | |
| Endotoxin | Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in the production of AAV, especially for applications in animal studies and gene therapy. Effective endotoxin quality control is essential in the development and manufacturing of AAV particles. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced AAV particles to ensure regulatory compliance. |
| Purity | AAV purity is critical for ensuring the safety and efficacy of AAV-based applications.AAV capsids are composed of three main protein components, known as viral proteins: VP1, VP2, and VP3. These proteins play a critical role in the structure and functionality of the AAV capsid. Monitoring the VP1, VP2, and VP3 content in AAV preparations is essential for quality control in AAV production. Our AAV particles are tested for showing three clear bands of VP1, VP2 VP3 by SDS-PAGE. |
| Sterility | The AAV virus samples are inoculated into the cell culture medium for about 5 days to detect bacterial and fungal growth. |
| Transducibility | Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of AAV to deliver genetic material into target cells or tissues, and assess gene expression and functional activities. |
| Empty vs. Full Capsids | Based-on our proprietary AAV production and purification technology, Creative Biogene can always offer AAV particles with high ratio of full capsids. If required, we can also assess the ratio for a specifc lot of AAV particles by transmission electron microscopy (TEM) or other methods. |
The suprachiasmatic nucleus (SCN) synchronizes circadian rhythms of behavior and physiology to the external light cycle. As the neuropeptide vasoactive intestinal polypeptide (VIP) is important for circadian light responses, researchers tested the hypothesis that rhythmic VIP-producing SCN neurons mediate circadian light responses in male and female mice. Using in vivo fiber photometry over multiple days, they found daily rhythms in spontaneous calcium events in SCN VIP neurons that peaked during subjective daytime and were disrupted by constant light. Light-evoked calcium responses peaked at subjective dusk and were more robust during subjective night. Using novel VIP sensor cells, researchers found that activity patterns in SCN VIP neurons were tightly coupled to spontaneous and NMDA-evoked VIP release. Finally, hyperpolarization of VIP neurons in vivo attenuated light-induced circadian changes in locomotion. These findings suggest that SCN VIP neurons exhibit circadian rhythms in both spontaneous and light-responsive activity and are essential for normal resetting of daily rhythms by ambient light.
To determine the circadian regulation of SCN VIP neuron activity over multiple days, researchers used long-term in vivo fiber photometry to record hourly spontaneous calcium activity in the SCN of freely moving mice (Figure 1a-d). Cre-dependent AAVs encoding the fluorescent calcium sensor GCaMP6s (AAV9-CAG-Flex-GCaMP6s) or a control fluorophore (AAV9-CAGFlex-EGFP) were injected into the SCN of VIP-IRES-Cre knock-in mice. Researchers found that daily rhythms in intracellular calcium levels and event frequency persisted for the number of days recorded, peaking at daily minimums in locomotor activity during light/dark (LD) cycles or constant darkness (DD) (Figure 1e-j). The amplitude of the rhythm was greatly disrupted in constant light (LL) (Figure 1i, j). These results suggest that SCN VIP neurons exhibit in vivo daily rhythms in spontaneous calcium activity.
Figure 1. SCN VIP neurons exhibit daily rhythms in spontaneous calcium activity in vivo. (Jones J R, et al., 2018)
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