Cat.No. : CSC-RR0148 Host Cell: U87
| Cat. No. | CSC-RR0148 |
| Description | U87 is a human primary glioblastoma cell line formally known as U-87 MG. It has epithelial morphology, and was obtained from a stage four 44 year-old cancer patient. The GFP Stable Cell Line-U87 constitutively expresses GFP. |
| Host Cell | U87 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Reporter Type | Fluorescent protein |
| Application | 1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Storage | Liquid nitrogen |
| Recommended Medium | Inquiry for instruction of culturing |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Glioma is one of the most common kinds of adult primary brain cancer, with a high mortality rate due to its location and significant invasion potential. The researchers found elevated GBP2 expression in glioma samples. Depletion prevents cell growth and migration, whereas overexpression promotes these processes. GBP2 modulates EGFR signaling and interacts with KIF22, which influences glioma growth both in vitro and in vivo. This work provides fresh insights into glioma biology, which could help guide future treatments.
Figure 1. The researchers examined the effects of GFP-GBP2 overexpression in U87 cells. The methods used to confirm GBP2 overexpression were RT-qPCR and Western blotting. Functional experiments (CCK8, colony formation, cell cycle, apoptosis, and Transwell invasion) demonstrated increased proliferation, migration, and altered cell cycle progression. The experiments were repeated at least three times. (Ren Y, et al., 2022)
A: By leveraging the GFP fluorescence, researchers can conduct live imaging to visualize and quantify the dynamic processes of cell migration and invasion using in vitro assays like scratch wound healing and Matrigel invasion chambers. This enables the study of how glioblastoma cells navigate through extracellular matrices and respond to chemotactic signals.
A: An ideal setup would involve co-culturing GFP-labeled U87 cells with endothelial cells to form a vascularized tumor model. Treatment with anti-angiogenic agents can then be monitored using fluorescence microscopy to assess changes in tumor vasculature and cell viability, providing insights into the therapy's effectiveness in disrupting the tumor's blood supply.
A: Yes, the cell line can be used in conjunction with models of the blood-brain barrier to evaluate the delivery efficiency of nanoparticles or other drug carriers. The uptake of GFP-labeled U87 cells can be quantified post-treatment to determine the success rate of these systems in transporting therapeutic agents across the barrier.
A: Gene function can be studied by silencing or overexpressing target genes in GFP-labeled U87 cells and observing changes in fluorescence intensity as a proxy for cell survival and proliferation rates. Techniques such as siRNA knockdown or CRISPR-Cas9 gene editing can be applied, followed by fluorescence-based assays to assess the impact on cellular behaviors.
A: The stable GFP expression allows for the formation of fluorescent tumor spheroids, which can be continuously monitored under a fluorescence microscope. This approach enables the quantitative analysis of spheroid size and growth over time, providing a 3D model to study glioblastoma cell-cell interactions, hypoxia, and drug responses within a more physiologically relevant environment.
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The GFP Stable Cell Line-U87 adapts to various growth conditions, which facilitates its use across different experimental setups. This adaptability means we can work with this cell line in a broad range of environments and protocols.
GFP Stable Cell Line-U87 has a defined tumorigenic potential, making it particularly valuable for studying tumor development and progression. This characteristic allows for consistent results in experiments focused on oncology and neurology.
This cell line provides consistent GFP fluorescence, which is crucial for reliable imaging and analysis. Consistent expression reduces variability and enhances the integrity of experimental findings.
The anchorage-dependent growth of GFP Stable Cell Line-U87 allows for straightforward cultivation and maintenance in standard tissue culture flasks, reducing the complexity of cell culture work.
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