Cat.No. : CSC-RR0109 Host Cell: B16-F10
| Cat. No. | CSC-RR0109 |
| Description | The B16-F10(Mouse) cell line was isolated from the skin of mouse with melanoma. The GFP Stable Cell Line-B16-F10(Mouse) constitutively expresses GFP. |
| Background | The development of GFP Stable Cell Line-B16-F10(Mouse) stems from the groundbreaking discovery of green fluorescent protein (GFP) in the 1960s. GFP |
| Host Cell | B16-F10 |
| Host Cell Species | Mus musculus (Mouse) |
| Stability | Validated for at least 10 passages |
| Reporter Type | Fluorescent protein |
| Application | 1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Storage | Liquid nitrogen |
| Recommended Medium | Inquiry for instruction of culturing |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Despite growing evidence of cancer stem cell (CSC) niches' crucial role in solid tumor progression within the tumor microenvironment (TME), many molecular players in this regulation remain unidentified. Researchers demonstrate that pharmacological β3-AR blockade in B16 melanoma-bearing mice reduces CSC marker expression and induces hematopoietic differentiation in the TME. β3-AR antagonist SR59230A promotes lymphoid/HSC and myeloid progenitor/HSC ratios, increases Ter119 and NK precursor cells, and enhances granulocyte precursors, indicating active hematopoiesis within tumors. Moreover, it induces MSC differentiation into adipocytes, potentially curbing melanoma progression by diminishing stemness traits.
Figure 1. Tumor growth rates were evaluated by researchers in C57BL/6 mice injected with B16-F10 cells, under control, vehicle, propranolol, or SR59230A treatment. Annexin V/PI staining, PI staining using confocal microscopy, Ki-67 immunohistochemical staining, and β2- and β3-ARs immunohistochemical staining in tumor tissues were also examined. The murine B16-F10 melanoma GFP stable cell line was acquired from Creative Biogene. (Calvani M, et al., 2020)
A: Factors influencing the selection of B16-F10 cells for establishing the GFP stable cell line included their relevance to cancer research and their suitability for studying cellular processes such as migration and invasion. Additionally, B16-F10 cells are well-characterized and widely used in tumor biology studies.
A: Verification and maintenance of stability and expression level of GFP in the B16-F10 stable cell line involved stable transfection, antibiotic selection, and validation of expression using fluorescence microscopy or flow cytometry. Maintenance included regular subculture and monitoring of fluorescence intensity to ensure stable expression levels.
A: Functional characterization of GFP in the B16-F10 stable cell line involved assessing its responsiveness to regulatory elements by monitoring changes in fluorescence intensity in response to stimuli. Additionally, GFP's involvement in cellular processes such as protein localization, organelle tracking, or cell cycle dynamics was investigated using live-cell imaging and fluorescence microscopy.
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An invaluable resource! The GFP Stable Cell Line has significantly enhanced my research capabilities, offering a reliable tool for studying tumor biology and evaluating therapeutic interventions in mouse models.
Consistent expression! This cell line surpasses expectations, providing bright and uniform GFP fluorescence, facilitating accurate quantification and imaging of tumor cells in vivo. Its stable GFP expression simplifies experimental workflows, allowing for efficient data collection and analysis, and accelerating discoveries in cancer research.
Reliable fluorescence! The GFP Stable Cell Line in B16-F10 cells offers stable expression of GFP, enabling clear visualization and tracking of cells in my mouse tumor model research. With the GFP Stable Cell Line, I can monitor tumor growth, metastasis, and response to treatment with confidence, advancing our understanding of cancer biology and therapy.
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