Human NPR3 Knockout Cell Line-HeLa
Cat.No. : CSC-RT1570
Host Cell: HeLa Target Gene: NPR3
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
Inquire for Price
Cell Line Information
Cell Culture Information
Safety and Packaging
| Cat. No. | CSC-RT1570 |
| Cell Line Information | A stable cell line with a homozygous knockout of human NPR3 using CRISPR/Cas9. |
| Target Gene | NPR3 |
| Host Cell | HeLa |
| Shipping | 10^6 cells/tube |
| Storage | Liquid nitrogen |
| Species | Human |
| Gene ID | 4883 |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:4-1:6. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Inquiry
Background
Applications
The natriuretic peptide receptor 3 (NPR3) gene, also known as NPRC, is an important component of the natriuretic peptide receptor family and plays an important role in cardiovascular homeostasis. The NPR3 gene encodes the NPR-C receptor, a cell surface protein involved in the clearance of circulating natriuretic peptides (NPs), thereby regulating their biological effects. Unlike other natriuretic peptide receptors (NPR-A and NPR-B), which are associated with guanylate cyclase activity and cGMP production, NPR-C primarily functions by removing active peptides and regulating their concentrations in the bloodstream.
The structure of NPR3 includes an extracellular ligand-binding domain, a single transmembrane domain, and a short intracellular domain. The role of this receptor in cardiovascular health extends to influencing vasodilation, diuresis, and sodium excretion. In addition to its classical roles, recent studies have also delved into the function of NPR3 in adipose tissue, where it affects the adipokine network and energy metabolism, suggesting a possible involvement in metabolic disorders. Mutations or dysregulation of the NPR3 gene can lead to various pathological conditions. Reduced NPR3 expression has been associated with hypertension, heart failure, and other cardiovascular diseases, highlighting its potential as a therapeutic target.
Here are some of the key applications of the Human NPR3 Knockout Cell Line - HeLa:
Cardiovascular Research: The NPR3 gene plays a key role in regulating blood pressure and heart function by regulating natriuretic peptides. The NPR3 Knockout HeLa cell line can be used to study the pathways and mechanisms underlying cardiovascular disease, helping to identify potential biomarkers and therapeutic targets.
Cancer Research: Since HeLa cells are derived from cervical cancer, the NPR3 knockout variant can be used to explore the role of NPR3 in tumorigenesis, proliferation, and metastasis.
Pharmacological Testing: The NPR3 knockout cell line serves as a valuable model for testing the efficacy and safety of drugs targeting the natriuretic peptide pathway.
Genomic Studies: These cells can be used to study the effects of NPR3 gene deletion on the entire genomic and transcriptomic landscape.
Signal Transduction Studies: By eliminating NPR3, researchers can dissect the role of this receptor in various signaling pathways.
For research use only. Not intended for any clinical use.
