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Quick Ligation Kit

Cat.No.
TDK-L002
Quick Ligation Kit
Description
This kit contains T7 Endonuclease I and buffer. T7 Endonuclease I recognizes and cleaves mismatch–specific DNA, even single base pair mismatches, cruciform DNA structures, Holliday structures or junctions, heteroduplex DNA and more slowly, nicked double-stranded DNA. The cleavage site is at the first, second or third phosphodiester bond that is 5' to the mismatch.
Applications
Determine genome targeting efficiency by digesting annealed PCR products with T7 Endonuclease I, the most application is the Crispr/cas9 monitor test.
Storage
Store at -20°C.

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